effect of a-769662, a direct ampk activator, on tlr-4 expression and activity in mice heart tissue
نویسندگان
چکیده
objective(s): tlr-4 activates a number of inflammatory signaling pathways. also, ampk could be involved in anti-inflammatory signaling. the aim of this study was to identify whether stimulation of ampk could inhibit lps-induced tlr-4 gene expression in mice hearts. materials and methods: heart ampk activity and/or tlr-4 expression was stimulated in different mice groups, using respectively ip injection of a-769662 (10 mg/kg) and lps (2 mg/kg) or a combination of both agents. moreover, compound-c (20 mg/kg), as an ampk antagonist, was intraperitoneally co-administrated with both a-769662 and lps in another group to investigate the role of ampk activity on tlr-4 regulation. after 8 hr, in addition to peripheral neutrophil cell count, myocardial p-ampk, p-acc as well as myd88 protein contents and tlr-4 expression was assessed by western blotting and real-time qrt-pcr, respectively. tnf-α and il-6 expression levels were also determined by elisa. results: lps induced heart tlr-4 expression (p<0.001) associating with an increase in the myocardial myd88 protein content (p<0.001), elevation of heart tnf-α (p<0.01) and il-6 (p<0.05) concentrations, and rise in the peripheral neutrophil cell count (p<0.001). administration of a-769662 decreased lps-induced tlr-4 expression (p<0.01) and alleviated peripheral neutrophil cell count (p<0.01). the inhibitory effect of a-769662 on lps-induced tlr-4 expression was reversed by antagonizing ampk with compound-c (p<0.001) which reduced p-ampk (p<0.05) and p-acc (p<0.01) myocardial protein contents in the lps+a-769662 group. conclusion: this study demonstrated that activation of ampk, by a-769662 agent, could inhibit tlr-4 expression and activity, suggesting a link between ampk and tlr-4 in heart tissue.
منابع مشابه
Effect of A-769662, a direct AMPK activator, on Tlr-4 expression and activity in mice heart tissue
Objective(s): TLR-4 activates a number of inflammatory signaling pathways. Also, AMPK could be involved in anti-inflammatory signaling. The aim of this study was to identify whether stimulation of AMPK could inhibit LPS-induced Tlr-4 gene expression in mice hearts. Materials and methods: Heart AMPK activity and/or Tlr-4 expression was stimulated in different mice groups, using respectively IP i...
متن کاملEffect of A-769662, a direct AMPK activator, on Tlr-4 expression and activity in mice heart tissue
OBJECTIVES TLR-4 activates a number of inflammatory signaling pathways. Also, AMPK could be involved in anti-inflammatory signaling. The aim of this study was to identify whether stimulation of AMPK could inhibit LPS-induced Tlr-4 gene expression in mice hearts. MATERIALS AND METHODS Heart AMPK activity and/or Tlr-4 expression was stimulated in different mice groups, using respectively IP inj...
متن کاملeffect of sub-grid scales on large eddy simulation of particle deposition in a turbulent channel flow
چکیده ندارد.
15 صفحه اولThe effect of 4 weeks of endurance training and eugenol consumption on the expression of Gap43 and E-cadherin genes in the heart tissue of chlorpyrifos-poisoned mice
1.Introduction Among the various tissues of the body, the heart is one of the main and most important organs of the body to which human life depends, and any tissue and physiological damage to it disrupts human health (10) . On the other hand, today, prescribing sports activities is accepted as an effective prescription in the prevention and treatment of many diseases and improving heart f...
متن کاملthe effects of multiple intelligences (focus on musical, visual, and linguistic) and direct instruction on learning grammar: a case on iranian efl students at elementary level
1.0 overview it seems that grammar plays a crucial role in the area of second and foreign language learning and widely has been acknowledged in grammar research. in other words, teaching grammar is an issue which has attracted much attention to itself, and a lot of teachers argue about the existence of grammar in language teaching and learning. this issue will remind us a famous sentence f...
a study on rate making and required reserves determination in reinsurance market: a simulation
reinsurance is widely recognized as an important instrument in the capital management of an insurance company as well as its risk management tool. this thesis is intended to determine premium rates for different types of reinsurance policies. also, given the fact that the reinsurance coverage of every company depends upon its reserves, so different types of reserves and the method of their calc...
منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of basic medical sciencesجلد ۱۹، شماره ۱۲، صفحات ۱۳۰۸-۱۳۱۷
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023